名古屋大学農学国際教育研究センター 研究室
研究室紹介
実践アフリカ開発研究室では、アフリカのサハラ砂漠より南（サブサハラアフリカ）における作物生産の向上と安定化に貢献するため、作物遺伝資源の環境応答や栽培技術開発に関する研究および研究成果に基づく社会実装の実現に向けた活動に取り組んでいます。
サブサハラアフリカのイネ生産性向上に向けた栽培学的研究
サブサハラアフリカにある多くの国では、コメの需要増加に対して国内生産が追いついていません。コメ増産はサブサハラアフリカの食糧安全保障にとって重要な課題です。イネの生産性を向上するためには、様々な生物的・非生物的ストレスの影響を受ける現地の栽培環境に応じた品種改良と適切な栽培技術の開発が必要です。実践アフリカ開発研究室では、共同研究のために構築したケニア農畜産業研究機構の拠点を活用し、現地の栽培環境と現行栽培技術の解析、現地に適した新品種の開発、様々な有用形質を有するイネ品種の環境応答と形質発現の評価、品種の能力を引き出す栽培技術の開発、技術普及のための社会経済的条件の解明など、サブサハラアフリカの稲作の安定化と生産性向上の実現に向けた総合的な取り組みを行っています。実践アフリカ開発研究室が中心となって実施しているアフリカ稲作開発に関連する研究プロジェクトの詳細については、以下のホームページをご覧ください。
有用遺伝子導入イネ系統を使ってケニアで栽培試験を行っています。
ケニアでの圃場試験は現地スタッフと協力して実施します。
ラボでの実験も行います。
必要に応じてフィールド調査も行います。
食糧援助（KR）の見返り資金、SATREPS（JICA/JST）、aXis（JST）などにより整備した、ケニア農畜産業研究機構ムエア支所の研究施設（ここを拠点にして共同研究を行っています）。
aXis（JST）によりケニアの3か所に整備した試験用水田を利用して多環境栽培試験を実施しています。
人工ストリゴラクトンを用いた根寄⽣雑草「ストライガ」防除技術の開発
根寄生雑草「ストライガ」は、ソルガム、トウモロコシ、陸稲等の主要穀物に寄生し、アフリカの穀物生産に甚大な被害をもたらしています。ストライガの種子は宿主植物の根から放出されるストリゴラクトンと呼ばれる植物ホルモンに反応して発芽します。しかし、宿主に寄生しないと生存できません。ストライガのこのような性質を利用して、土壌中のストライガ種子に人工ストリゴラクトンを施用し、強制的に発芽させて枯死させる「自殺発芽」と呼ばれる方法が注目されてきました。実践アフリカ開発研究室では、共同研究者が開発した安価で、極めて高い活性を持ち、ストライガの発芽にだけ作用する革新的な化合物「スフィノラクトン-7（SPL7）」を利用したストライガ防除技術の開発に向けて、ケニアの圃場におけるSPL7によるストライガ発芽誘導の実証、効果的な施用方法の検討、ストライガの遺伝的多様性の評価、被害実態の解明などに学内外の研究者と共同で取り組んでいます。
ストライガに寄生されたトウモロコシ畑
メンバー
※カッコ内は、内数で留学生の人数を示す。
業績
原著論文
39. Asano, K., Azhar, A., Audia, F., Murano, H., Naito, H., Abbas, B., Romkaew, J., Sinma, K., Makihara, D. and Ehara, H.
Changes in photosynthetic performance and dry matter production in sago palm seedlings with the application of different nutrient levels
Tropical Agriculture and Development 67(3) : 90-97 (2023.12.22)
38. Mayumi Kikuta, Daniel M. Menge, Emily W. Gichuhi, Hiroaki Samejima, Rena Tomita, John M. Kimani, Ruth N. Musila, Kazuyuki Doi, Motoyuki Ashikari, Rosalyn Angeles-Shim, Kshirod K. Jena, and Daigo Makihara
Contribution of genes related to grain number (Gn1a and WFP) introgressed into NERICA 1 to grain yield under tropical highland conditions in central Kenya
Plant Production Science, Plant Production Science, 26(3): 309-319 (2023.8.8)
DOI: 10.1080/1343943X.2023.2245127
37. Kagito, S., Kikuta, M., Samejima, H., Gweyi-Onyango, J. P., Gikonyo, E. W., Gichuhi, E. W., Menge, D. M., Kimani, J. M., Yamauchi, A. and Makihara, D.
Yield responses of upland rice varieties to low N conditions in central Kenya
Journal of Agricultural Science, 15(6), 19-29 (2023.5.15)
36. Tomita, R., Reyes, V. P., Fukuta, Y., Gichuhi, E. W., Kikuta, M., Menge, D. M., Doi, K. and Makihara, D.
Genetic variation of blast (Pyricularia oryzae Cavara) resistance in the longistaminata chromosome segment introgression lines (LCSILs) and potential for breeding use in Kenya
Plants, 12(4), 863 (2023.2.14)
35. Reyes, V. P., Kitony, J. K., Nishiuchi, S., Makihara, D. and Doi, K.
Utilization of genotyping-by-sequencing (GBS) for rice pre-breeding and improvement: a review
Life, 12(11), 1752. (2022.11.1)
34. Gorfer, M., Borruso, L., Deltedesco, E., Gichuhi, E. W., Menge, D. M., Makihara, D., Praeg, N., Cesco, S., Mimmo, T., Merbold, L. and Leitner, S.
The effect of environmental parameters and fertilization practices on yield and soil microbial diversity in a Kenyan paddy rice field
Applied Soil Ecology, 176, 104495, (2022.5.9)
DOI: 10.1016/j.apsoil.2022.104495
33. Kakehashi, T., Kikuta, M., Menge, D. M., Gichuhi, E. W., Samejima, H. and Makihara, D.
Alternate wetting and drying (AWD) mitigates the decline in grain filling of Basmati 370 due to low temperature in tropical highlands
Agronomy, 11(11), 2345, (2021.11.19)
32. Aidil Azhar, Fitri Audia, Koki Asano, Daigo Makihara, Hitoshi Naito, Daisuke Sugiura, Hiroshi Ehara
The impact of different soil water levels on nitrogen, phosphorus, and potassium uptake, photosynthetic performance, and sugar distribution of sago palm seedlings
Agrosystems, Geosciences & Environment 2021;4:e20191 (2021.7.24)
31. Hiroaki SAMEJIMA, Keisuke KATSURA, Mayumi KIKUTA, Symon Mugo NJINJU, John Munji KIMANI, Akira YAMAUCHI and Daigo MAKIHARA
Factors explaining differences in yield response to high nitrogen fertilization among rice varieties under tropical highland conditions in central Kenya
JARQ, 55(3): 209-216. (2021.7.1)
30. Hasegawa, T., Wainaina, C. M., Shibata, A., Lucob-Agustin, N., Makihara, D., Kikuta, M., Menge, D. M., Gichuhi, E. W., Samejima, H., Kano-Nakata, M., Kimani, J. M., Musila, R. N., Yamauchi, A. and Inukai, Y.
The outstanding rooting1 mutation gene maintains shoot growth and grain yield through promoting root development in rice under water deficit field environments.
Journal of Agronomy and Crop Science (2021.6.17)
29. Vincent Pamugas Reyes, Rosalyn B. Angeles-Shim, Merlyn S. Mendioro, Ma.Carmina C. Manuel, Ruby S. Lapis, Jung Hyun Shim, Hidehiko Sunohara, Shunsaku Nishiuchi, Mayumi Kikuta, Daigo Makihara, Kshirod K Jena, Motoyuki Ashikari, Kazuyuki Doi
Marker-assisted introgression and stacking of major QTLs controlling grain number (Gn1a) and number of primary branching (WFP) to NERICA cultivars
Plants, 10(5): 844 (2021.4.22)
28. Aidil Azhar, Makihara D., Naito N., Asano K., Takagi M., Unoki S., Tomita R., Abbas B. & Ehara H.
Sago palm (Metroxylon sagu Rottb.) response to drought condition in terms of leaf gas exchange and chlorophyll a fluorescence
Plant Production Science, published online. (2020.8.17)
DOI: 10.1080/1343943X.2020.1794914
27. Samejima H., Kikuta M., Katura K., Daniel M. Menge, Emily W. Gichuhi, Cornelius M. Wainaina, John M. Kimani, Inukai Y., Yamauchi A., and Makihara D.
A method for evaluating cold tolerance in rice during reproductive growth stages under natural low-temperature conditions in tropical highlands in Kenya
Plant Production Science 23: 466-476 (2020.6.16)
DOI: 10.1080/1343943X.2020.1777877
26. Daniel M. Menge, Kano-Nakata M., Yamauchi A., Roel R. Suralta and Makihara D.
Root and shoot responses of upland New Rice for Africa varieties to fluctuating soil moisture conditions as affected by different levels of nitrogen fertilization
Journal of Agronomy and Crop Science, 206:322-337 (2020.6)
25. Kikuta M., Makihara D., Pasolon Y.B., Rembon F.S., Miyazaki A. and Yamamoto Y.
Agronomic traits and grain quality of upland rice cultivated in southeast Sulawesi, Indonesia
Journal of International Cooperation for Agricultural Development 18: 41-47. (2020.3)
24. Samejima H., Katsura K., Kikuta M., Symon M. NJINJU, John M. KIMANI, Yamauchi, A., and Makihara D.
Analysis of rice yield response to various cropping seasons to develop optimal cropping calendars in Mwea, Kenya.
Plant Production Science, 23(3): 297-305 (2020.2.13)
DOI: 10.1080/1343943X.2020.1727752
23. Azhar A., Makihara D., Naito H. and Ehara H.
Evaluating sago palm (Metroxylon Sagu Rottb.) photosynthetic performance in waterlogged conditions: utilizing pulse-amplitude-modulated (PAM) fluorometry as a waterlogging stress indicator.
Journal of the Saudi Society of Agricultural Sciences 19(1): 37-42. (2020.1)
DOI: 10.1016/j.jssas.2018.05.004
22. Yoshimichi Fukuta, Tomohisa Suzuki, Seiji Yanagihara, Mitsuhiro Obara, Asami Tomita, Ryo Ohsawa, Catherine Wanjiku Machungo, Nagao Hayashi, Daigo Makihara
Genetic variation in blast (Pyricularia oryzae Cavara) resistance in rice (Oryza sativa L.) accessions widely used in Kenya
Breeding Science, 69(4): 672-679(Print ISSN : 1344-7610, Online ISSN: 1347-3735) (2019.11.9)
21. Fukuta, Y., Telebanco-Yanoria, M. J., Hayashi, N., Yanagihara, S., Machungo, C. W. and Makihara, D.
Pathogenicities of rice blast (Pyricularia oryzae Cavara) isolates from Kenya
Plant Disease, 103(12): 3181-3188(ISSN: 0191-2917, e-ISSN: 1943-7692) (2019.10.21)
DOI: 10.1094/PDIS-04-19-0870-RE
20. Phung, H. D., Sugiura, D., Sunohara, H., Makihara, D., Kondo, M., Nishiuchi, S. and Doi, K.
QTL analysis for carbon assimilate translocation-related traits during maturity in rice (Oryza sativa L.)
Breeding Science, 69(2): 289-296(Online ISSN 1347-3735, Print ISSN 1344-7610) (2019.6.5)
19. Magoti R., Kikuta, M., Chemining'wa, G., Kinama, J., Kimani, J., Samejima, H., Gicheru, P. and Makihara, D.
Growth of rice varieties in different Kenyan soil types under water-deficit conditions.
Journal of Agricultural Science, 11, 1-11(Print ISSN 1916-9752, Online ISSN 1916-9760) (2019.5.15)
18. Hasegawa, T., Shibata, A., Wainaina, C. M., Lucob-Agustin, N., Makihara, D., Samejima, H., Kikuta, M., Menge, D. M., Kimani, J. M., Yamauchi, A. and Inukai, Y.
Evaluation of a newly identified mutation gene that promotes root elongation for improvement of drought avoidance in rice
Journal of International Cooperation for Agricultural Development 17: 34-40. (2019.3)
17. Menge, D., Onyango, J. C., Yamauchi, A., Kano-Nakata, M., Asanuma, S., Tran, T. T., Inukai, Y., Kikuta, M., and Makihara, D.
Effect of nitrogen application on the expression of drought-induced root plasticity of upland NERICA rice
Plant Production Science, 22, 180-191 (2019.1.21)
DOI: 10.1080/1343943X.2018.1561194
16. Kikuta, M., Makihara, D., Arita N., Miyazaki, A. and Yamamoto, Y.
Effects of different water management practices on the dry matter production process and characteristics in NERICAs
Plant Production Science, 22, 168-179 (2019.1.11)
DOI: 10.1080/1343943X.2018.1561195
15. Mayumi Kikuta, Yoshinori Yamamoto, Yulius Barra Pasolon, Fransiscus Suramas Rembon, Akira Miyazaki, and Daigo Makihara
Effects of slope-related soil properties on upland rice growth and yield under slash-and-burn system in South Konawe Regency, Southeast Sulawesi Province, Indonesia.
Tropical Agriculture and Development, 62, 60-67. (Online ISSN 1882-8469, Print ISSN 1882-8450) (2018.10.12)
14. Stella Owusu-Nketia, Yoshiaki Inukai, Satomi Ohashi, Roel Suralta, Kazuyuki Doi, Shiro Mitsuya, Mana Kano-Nakata, Jonathan Niones, Dinh Thi Ngoc Nguyen, Takuya Kabuki, Daigo Makihara, Akira Yamauchi.
Root plasticity under fluctuating soil moisture stress exhibited by backcross inbred line of a rice variety, Nipponbare carrying introgressed segments from KDML105 and detection of the associated QTLs.
Plant Production Science 21(2): 106-122. (2018.5.9)
DOI: 10.1080/1343943X.2018.1446759
13. Symon M. Njinju, Hiroaki Samejima, Keisuke Katura, Mayumi Kikuta, Joseph P. Gweyi-Onyango, John M. Kimani, Akira Yamauchi, and Daigo Makihara
Grain yield responses of lowland rice varieties to increased amount of nitrogen fertilizer under tropical highland conditions in central Kenya.
Plant Production Science, 21, 59-70. (2018.2.28)
DOI: 10.1080/1343943X.2018.1436000
12. Cornelius Mbathi Wainaina, Daigo Makihara, Mitsuru Nakamura, Akihiro Ikeda, Taro Suzuki, Yuko Mizukami, Toshihiro Nonoyama, Kazuyuki Doi, Mayumi Kikuta, Hiroaki Samejima, Daniel Makori Menge, Akira Yamauchi, Hidemi Kitano, John Munji Kimani, Yoshiaki Inukai.
Identification and validation of QTLs for cold tolerance at the booting stage and other agronomic traits in a rice cross of a Japanese tolerant variety, Hananomai and a NERICA parent, WAB56-104
Plant Production Science 21(2): 132-143. (2018.2.21)
DOI: 10.1080/1343943X.2018.1440970
11. Aidil Azhar, Daigo Makihara, Hitoshi Naito and Hiroshi Ehara.
Photosynthesis of sago palm (Metroxylon sagu Rottb.) seedling at different air temperatures.
Agriculture, 8, 4 (2018.1.1)
DOI: 10.3390/agriculture8010004
10. Kundu, C. A., Ishii, M., Sato, K., Wanjogu, R. K., Makihara, D., Yamauchi, A. and Masunaga, T.
An assessment of paddy production system in central Kenya with special reference to micronutrients.
Journal of Agricultural Science 9: 49-63. (2017.5.15)
9. Kikuta, M., Yamamoto, Y., Pasolon, Y. B., Rembon, F. S., Miyazaki, A. and Makihara, D.
How growth and yield of upland rice vary with topographic conditions:
A case of slash-and-burn rice farming in south Konawe Regency, southeast Sulawesi province, Indonesia.
Tropical Agriculture and Development, 60, 162-171.(Online ISSN : 1882-8469, Print ISSN : 1882-8450) (2017.3.20)
8. Wainaina, C. M., Makihara, D., Samejima, H., Kikuta, M., Menge, D. M., Kimani, J. M. and Inukai, Y.
Development of a new cultivation technology for cold stress escape through flowering time manipulation by water management in the highlands of east Africa
Journal of International Cooperation for Agricultural Development 15: 33-42 (2017.3)
7. Kikuta, M., Makihara, D., Arita N., Miyazaki, A. and Yamamoto, Y.
Growth and yield responses of upland NERICAs to variable water management under field conditions.
Plant Production Science, 20, 36-46. (2016.11.9)
DOI: 10.1080/1343943X.2016.1245102
6. Kundu, C. A., Ishii, M., Sato, K., Masunaga, T., Wanjogu, R. K., Njagi, R. E., Yamauchi, A. and Makihara, D.
Evaluation of soil chemical properties under paddy production system in central Kenya: soil exchangeable cations.
Journal of Agricultural Science, 8, 136-148. (2016.7.15)
5. Menge, D., Kameoka, E., Kano-Nakata, M., Yamauchi, A., Asanuma, S., Asai, H., Kikuta, M., Suralta, R.R., Koyama, T., Tran, T. T., Siopongco J. D .L. C., Mitsuya, S., Inukai, Y. and Makihara, D.
Drought-induced root plasticity of two upland NERICA varieties under conditions with contrasting soil depth characteristics.
Plant Production Science, 19, 389-400. (2016.2.29)
DOI: 10.1080/1343943X.2016.1146908
4. 齋藤元也、小川茂男、槇原大悟、浅沼修一
ケニアにおける畑地利用による米増産の可能性。
Estimation of potential area for upland rice production in Kenya
畑地農業、676、9-22 (2015.3)
3. Wainaina, C. M., Inukai, Y., Masinde, P. W., Ateka, E. M., Murage, H., Kano-Nakata, M., Nakajima, Y., Terashima, T., Mizukami, Y., Nakamura, M., Nonoyama, T., Saka, N., Asanuma, S., Yamauchi, A., Kitano, H., Kimani, J. and Makihara, D.
Evaluation of cold tolerance in NERICAs compared with Japanese standard rice varieties at the reproductive stage.
J. Agron. Crop Sci., 201, 461-472.(Print ISSN:0931-2250) (2015.2.27)
2. Sakurai, T., Nasuda, A., Murage, H. and Makihara, D.
Impact of adopting a new cash crop: a randomized rice seed provision trial in the Kenyan highlands.
The Japanese Journal of Rural Economics, 16, 25-32. (Online ISSN:2189-5880, Print ISSN:2187-946X) (2014.3)
1. Nasuda, A., Sakurai, T., Murage, H. and Makihara, D.
Journal of International Cooperation for Agricultural Development, 13, 29-37.(Online ISSN:2436-2786) (2014.3)
著書・総説・その他
7. 槇原大悟, 伊藤香純, 浜野充, 菊田真由実, 松川みずき, 栗田明華, 長谷川友美, 江原宏
農学分野の国際協力キャリアパスについて考える. 農学分野で国際協力を職業にしたいなら
-先人からの具体的なアドバイス- 犬飼義明・仲田（狩野）麻奈 編
アイペック，東京．pp. 51-65. (2021.4)
6. 土井一行・芦苅基行・菊田真由実・槇原大悟
イネ収量関連遺伝子の同定と利用―ケニアでの試み―
アグリバイオ（北隆館） 4(7): 18-22 (526-530)(Print ISSN:01327-07) (2020.6)
5. 槇原大悟
アフリカ農業における課題解決をめざす国際共同研究 アフリカ研究の現場から Vol.8
アフリカ 59(4)30-33 (2019)
4. 槇原大悟・江原宏
11.1 熱帯における農業・農村開発 p232-233、11. 熱帯農業と国際協力
11.2 農業国際開発における日本の役割 p233-237、11. 熱帯農業と国際協力
熱帯農学概論（江原宏・樋口浩和編、培風館、pp296. ISBN 978-4-563-07826-3.）(2019.1)
3. 槇原大悟
4.4 アフリカ p75-76、4. 農耕文化圏と熱帯各地の農業
11.4 今後の国際農業協力の方向性 p242-242、11. 熱帯農業と国際協力
熱帯農学概論（江原宏・樋口浩和編、培風館、pp296. ISBN 978-4-563-07826-3.）(2019.1)
2. Makihara, D., Kimani, J., Samejima, H., Kikuta, M., Menge, D., Doi, K., Inukai, Y., Maekawa, M., Masunaga, T., Sasaki, Y., Katsura, K., Kitano, H., Mitsuya, S., Kano-Nakata, M., Wainaina, C., Gichuhi, E., Njinju, S., Kagito, S., Magoti, R., Wakaria, M., Kundu, C., and Yamauchi A.
Development of rice breeding and cultivation technology tailored for Kenya's environment
Crop Production under Stressful Conditions - Application of Cutting-edge Science and Technology in Developing Countries.
Springer p27-47. Kokubun, M. and Asanuma, S. (Eds.)
(eBook ISBN:978-981-10-7308-3, Hardcover ISBN:978-981-10-7307-6) (2018.8.2)
DOI: 10.1007/978-981-10-7308-3_3
1. 槇原大悟
3.8 シアーバターノキ p100-105
作物栽培大系7 工芸作物の栽培と利用
（巽二郎 編、朝倉書店 pp 272. ISBN978-4-254-41507-0 C3361）(2017.7.5)